Determination of Genetic Polymorphism within Güney Karaman Sheep-Breed via RAPD-PCR Method

Determination of Genetic Polymorphism within Güney Karaman Sheep-Breed via RAPD-PCR Method

Aytekin, İbrahim
Boztepe, Saim

Analysis of individual genotypes aims to provide information for understanding within population genetic parameters such as genetic similarity, polymorphism and heterozygosity. The aims of this research were to determine the genetic parameters within Güney Karaman sheep involved in a protection programme of gene resources by using randomly oligonucleotide primers via Randomly Amplified Polymorphic DNA (RAPD) method. Among the primers tested, 10 most suitable ones, in terms of repeatability and scorability, were used and 1451 DNA fragments were scored. All the RAPD fragments were determined to be between 600 - 3000 bp in size. From 147 fragments, 133 were polymorphic (90.48%) and 14 fragments were monomorphic (9.52%). Within population average genetic similarity (Fragment Sharing Frequency, Fxy) and genetic distance were calculated as 0.7001 and 0.2991, respectively. The average expected heterozygosity was estimated as 0.3273 ± 0.1697.

Conference or Workshop Item
PeerReviewed

2010-06

374