A Study on Micropropagation as a Tool for Sustainable Utilization of Jujube (Zizyphus jujuba Mill.) Genotypes

A Study on Micropropagation as a Tool for Sustainable Utilization of Jujube (Zizyphus jujuba Mill.) Genotypes

SAN, Bekir
YILDIRIM ADNAN, Nurhan
YILDIRIM, Fatma
MUSTAFA ECEVIT, Fevzi

Micropropagation is as a tool for sustainable utilization and allows the production of a large number of virus-free clones within a short period. Also, micropropagationis fundamental for the conservation of genetic resources. The presentstudy was carried out to establish protocols for the invitropropagation of Jujube (Ziziphusspp)and contribute to the conservation of plant geneticresources.In the study, shoot tips of two selected jujube genotypes (20-C-10 and 20-C-22) were used as a material. MS (Murashige and Skoog) medium supplemented with both TDZ (thidiazuron) and BAP (benzylaminopurine) as a cytokinin was used for micropropagation of jujube genotypes. The highest percentage of explants forming shoots (93.3 %) and the highest number of shoot per explants (5.7) was obtained on the MS medium containing 0.1 mg/l TDZ+0.5 mg/l BAP+0.1 mg/l IBA (indolebutyric acid)+0.3 mg/l GA3(gibberellic acid) in 20-C-10 jujube genotype. The highest rooting percentages of 20-C-10 and 20-C-22 jujube genotypes (83.3 % and 80.0 %, respectively) were obtained on half-strength MS medium supplemented with 2.0 mg/l IBA. Keywords: jujube, micropropagation, thidiazuron, benzylaminopurine, in vitro rooting

Article
PeerReviewed

ISSN 2233 - 0054

International Burch University

2014-06

2752